Esterase activities of human carbonic anhydrases B and C.
نویسندگان
چکیده
منابع مشابه
Carbon dioxide hydration activity of carbonic anhydrase: kinetics of alkylated anhydrases B and C from humans (metalloenzymes-isoenzymes-active sites-mechanism).
A stop-flow kinetic study was performed on the carbon dioxide hydration activity of the human carbonic anhydrase B isoenzyme carboxymethylated at its histidine(200), and of the human C isoenzyme carboxyketoethylated at its histidine(63). The Michaelis-Menten parameters determined between pH 5.6 and 8.7 showed striking differences between the native and the alkylated enzymes, as well as between ...
متن کاملHuman Carbonic Anhydrases II. SOME PHYSlCOCHEMICAL PROPERTIES OF NATIVE ISOZYMES AND OF SIMILAR ISOZYMES
Detailed studies of a relatively large number of human erythrocyte carbonic anhydrases of different electrophoretic mobilities have shown that individually each is very similar to one of the major isozymes, either B or C. The enzymatic activities, the zinc, nitrogen, and amino acid contents, and the molecular weights of members of a given type are similar. Some differences in the tryptic peptid...
متن کاملHuman carbonic anhydrases. VI. Levels of isozymes in old and young erythrocytes and in various tissues.
The more acidic, minor isozymes of human carbonic anhydrase types B and C are present in higher concentrations in the older erythrocyte populations. The levels of all of these isozymes can be determined quantitatively by immunochemical methods following their separation by electiofocusing. A wide variety of human tissues have been assayed for carbonic anhydrases. Significant levels of the C typ...
متن کاملA Plant-Type (b-Class) Carbonic Anhydrase in the Thermophilic Methanoarchaeon Methanobacterium thermoautotrophicum
Carbonic anhydrase, a zinc enzyme catalyzing the interconversion of carbon dioxide and bicarbonate, is nearly ubiquitous in the tissues of highly evolved eukaryotes. Here we report on the first known plant-type (b-class) carbonic anhydrase in the archaea. The Methanobacterium thermoautotrophicum DH cab gene was hyperexpressed in Escherichia coli, and the heterologously produced protein was puri...
متن کاملPurification and properties of human erythrocyte carbonic anhydrases.
Three different methods are described for separating hemoglobins from carbonic anhydrases in hemolysates from human erythrocytes. The preferred method involves adsorption on diethylaminoethyl Sephadex at pH 8.7 followed by selective elution of the carbonic anhydrases. Carbonic anhydrases A, B, and C are subsequently separated from each other on DEAE-Sephadex by elution with 0.05 M Trischloride ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 242 18 شماره
صفحات -
تاریخ انتشار 1967